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To apply BETI to method validation requires that the true or a certified value of the analyte of interest is known [ 30].
To ensure the accuracy of the measurement, we included reference material from a dogfish (DORM-2; National Research Council of Canada, Ottawa, Ontario, Canada) with a certified value of 4.64 ± 0.26 μg/g in the analyses; the observed values fell within the certified range.
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The total arsenic determination was validated against the certified reference material CRM 7405-a (Hijiki) with a certified value for arsenic of 35.8 ± 0.9 μg As g 1; we obtained 35.6 ± 0.9 μg As g 1 (n = 3).
Hire a Certified Value Analyst (CVA) to determine the value of such assets.
If concentrations were significantly different from the certified value of SRM2670a, the instrument was recalibrated and the previous batch of samples was reanalyzed.
We ensured the accuracy by measuring certified reference material BCR 397 (IRMM, Geel, Belgium), where our result was 11.74 ± 0.87 μg/g, compared with the certified value of 12.3 ± 0.5 μg/g.
The measured lead concentration of the 955b blood SRM was 38.6 ± 1.3 μg/dL (2× SE, n = 5), in good agreement with the certified value of 39.4 μg/dL.
On the other hand, certified values of CRM 7902-a or CRM 7904-a were calculated from PCB concentrations in a PCB root solution determined by combinations of four kinds of GC column and GC/MS ionization modes (electron ionization (EI) and NCI [ 15]), PCB concentrations in CRM 7903-a or CRM 7905-a (oil matrix), and mass ratio between the PCB spike solution and oil matrix for the preparation.
Results showed that there was a good agreement between the recovered and certified values of the metal and metalloid contents in certified reference material.
Method validation was confirmed by the good agreement between the results of the proposed method and certified values of Cd, Cu, and Pb.
Table 3 summarizes the results obtained from voltammetric determination of l-serine and l-phenylalanine along with the certified values of the analyzed pharmaceutical products.
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