This method allowed us to detect very sensitively the presence or absence of a certain orientation of DNA, based on the presence or absence of PCR amplification product of different lengths.
Thus, the fact that only sgRNA pairs that supposedly leave a 5′ overhang can generate DSBs may reflect a physical encumbrance between the two nickases in a certain orientation when they are close on the target DNA (Nishimasu et al. 2014).
The complex 3D feather-like nanostructure has a hierarchical structure, which seems that new small branches with a certain orientation (still about 30°) will grow on the oblique branches.
Based on the charge transfer on the surface of carbon nanotubes (CNTs), the electrical resistance can be applied to evaluate the conducting efficiency in a certain orientation.
According to previous studies [22 24], in electroless silicon etching, SiNWs are synthesized with a certain orientation, determined by the crystallographic orientation of the wafer.
Consequently, a CNVR detected at a certain position and in a certain orientation within a contig might have a different position and orientation within this contig.
The direction of the field might force (bio- polymers into a certain orientation.
The influence of knowledge from a certain professional orientation on the perception of WTs is therefore evident.
A grid box, large enough to avoid biases of the docking results to a certain ligand orientation, was centered on the previously described heparin-binding site (H23, K25, R65, K69, K72 and R73; Kuschert et al. 1998).
The macrocomposite consists of resin and fibres with a certain fibre orientation distribution.
