The ability to express fluorescence intensity in quantitative terms provides a simple, centralized quality control component that incorporates all of the many factors impacting the optics of an assay.
A series of centralized quality controls after genotyping led to exclusion of 243 SNPs.
The key features of such a framework are: political and operational leadership, centralized quality standards and decentralized quality control processes, systematic learning, and adaptation to local circumstances, flexible human resource practices that sustain continuity of effort.
Genotype data for eight SNPs were available from genotyping conducted using the Illumina iSelect array as part of a large-scale project, the Collaborative Oncological Gene-environment Study (COGS) with thorough centralized quality control measures [ 24].
Centralized quality assurance was provided by UCSF.
Four different local A1C assays were prospectively subjected to centralized quality control: Mono-S HPLC method, Boehringer Mannheim Tinaquant, Roche Unimate, and Abbott IMx.
The main strengths of this study include the uniform genotyping procedures, stringent centralized quality controls and large sample size, which provides us with sufficient statistical power to detect associations between genetic variants with moderate effects and breast cancer prognosis.
Two independent investigators will enter the data in a centralized database; data quality will be assessed.
In this study, MRI was performed using uniform imaging sequences and a centralized setup for quality control and image restorations (Jack et al., 2008 a).
A centralized, blinded PCR respiratory quality control panel is also provided to all sites to ensure procedure validation on site before specimens are processed locally.
