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Five hundred microliters of non-osteogenic α-minimum essential medium (α-MEM: Gibco, Tokyo, Japan) supplemented with 10%% fetal bovine serum and 1 % penicillin/streptomycin was added in each well, and then MC3T3-E1 cells were seeded at a cells density of 3 × 104 cells/cm2 per sample of the scaffolds.
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Cells were seeded at a cell density of 1 * 104 cells per well and propagated for 24 h.
mOECs were seeded at a cell density of 15,000 cells per well into a 96-well microplate (Nunclon) in complete media.
mOECs were seeded at a cell density of 2000 cells per well in a CellCarrier-384 Ultra microplate (PerkinElmer) in 50 µL complete media.
mOECs were seeded in Corning 384-well spheroid microplates at a cell density of 5000 cells per well in 50 µL complete media.
Sf9 insect cells were infected with the virus at a cell density of 4.0 × 106 cells ml−1 in Sf900 II medium (Invitrogen) and grown for 48 h at 27 °C.
Taking into account these results, a double-bed system with a cell density of 33 cell cm−2 (210 c.p.s.i).i
For gene expression using the pET-15b vector, isopropyl-β-d-1-thiogalactopyranoside (IPTG) was added (final concentration 0.2 mM) at a cell density of 0.4 (optical density at 600 nm) to induce gene expression.
This gives a cell density of 1.47 g cm−3 which compares well with the experimentally determined unit cell density of 1.507 ± 0.2 g cm−3.
We report the engineering of a monolayer of flattened endothelial cells with a cell density of 966 ± 242 cells/mean(mean ± SD).
Maximal intra-day growth rates of 1.9 d−1 were observed at a cell density of 1 3 g L−1.
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