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In this report, a cell pattern was prepared on poly(dimethylsiloxane) (PDMS) substrate by vacuum ultraviolet (VUV) lithography.
We investigated the ability of the microscale topography of a three-dimensional (3-D) structure arrayed on the surface of a substrate to induce and maintain a cell pattern by controlling cell behavior.
Cell culture experiments using osteoblast-like cells demonstrated that these narrow V-shaped grooves had sufficient cell repellency to form and maintain a cell pattern on the surface for at least 14 days.
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Next, as a cell-patterning method, the protocols of coating substrates with the PEG lipid, irradiating a pattern of light onto the coated substrate, and loading cells onto the irradiated surface are described.
Small round to spindle-shaped cells arranged in a spindle cell pattern with finely granular chromatin, inconspicuous nucleoli, and a scant to moderate amount of eosinophilic cytoplasm were seen in the form of many scattered tumor foci (arrowheads).
Histologically, the tumor located just beneath the visceral pleura exhibited the proliferation of small round to spindle-shaped cells arranged in a spindle cell pattern with finely granular chromatin, inconspicuous nucleoli, and a scant to moderate amount of eosinophilic cytoplasm.
Layer-by-layer a 3D cell pattern was generated.
Because the dispensing technology can make a precise cell pattern directly by dispensing small droplets of biological materials containing living cells, it has advantages of simplicity and flexibility for fabrication of complex cellular structures.
These were 147 (3.81%) genes with apparently incomplete reprogramming, probably with a somatic cell pattern of expression.
All SEs presented a uniform cell pattern, and three cells type was displayed in 9/15 (60%) cases with SS (p = 0.011).
Genes were categorized as either predominant in the brain (Brain ), predominant in a stem cell pattern with or without expression elsewhere (Stem cell-like ), or possessing unique patterns (Various ).
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