Results showed that the cell limit of detection (LOD) and the cell limit of quantification (LOQ) were 2.64 × 104 cells and 9.86 × 104 cells per well of microplate, respectively.
Using a PCR based amplification strategy for the analysis of single cells, a limit of 80 copies per cell was reported [ 18].
These have apparently higher levels of Ins P6 than rat tissues; however, the difficulty of accurately measuring cellular wet weight, plus the absence of connective tissue in a cell culture, limit the interpretability of such comparisons.
While electrochemical reduction shows promise, several limitations were identified, an apparent requirement for low-salt conditions, a higher-than-optimal temperature (10 °C), and a current cell pressure limit of 50 bar.
Using a PCR based amplification strategy for single cell analysis, a limit of 80 copies per cell was reported to be the lower range for registration of two fold changes in input RNA [ 18].
Podocalyxin at the apical membrane, a phenotypic marker of the podocyte cell, limits the passage of negatively charged albumin [ 10].
The maximal size of a cell was limited to a sphere with 20 µm radius to obtain reasonable cell regions at the borders.
A wide linear response to cells ranging from 80 to 4 × 106 cells mL−1 with a detection limit of 40 cells mL−1 was obtained.
The experiments performed with Escherichia coli evidenced a linear response for concentrations ranging 102 108 cell ml−1, with a limit of detection of 55 cells ml−1 in PBS, without pre-enrichment steps.
In this paper, a linear relationship was obtained between the SWV peak current and the cell concentration in the range 2 × 102 2 × 108 cell mL−1 with a detection limit of 2 × 102 cell mL−1.
The linear range is from 0 to 500 cells mL−1 with a detection limit of 47 cells mL−1.
