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Stained cells were acquired on a BD LSR Fortessa cell analyzer and analyzed with FlowJo (Treestar) software.
Cells were then analyzed using a LSRFortessa cell analyzer (BD Biosciences), and data analyzed using FlowJo version 7.6.4 software.
Flow cytometry was performed on a Cyan flow cytometer (Dako, Denmark) with Summit software v4.3, or on a BD LSR Fortessa cell analyzer (BDBioscience) and analyzed with Flowjo.
Stained cells were then analyzed using a FACSCalibur cell analyzer (Becton Dickinson).
Flow cytometry was performed on a LSRFortessa cell analyzer (BD Biosciences), and data were analyzed with FlowJo 7.6.4 software (Treestar).
The cells were then incubated with 5 μl of annexin V FITC (eBioscience, Inc., San Diego, CA) and 5 μl of propidium iodide (eBioscience, Inc., San Diego, CA) at room temperature for 5 minutes in the dark before analyzing the cells on a FACSCalibur cell analyzer.
Five days after transduction cells were analyzed by flow cytometry using a BD FACSCalibur cell analyzer (BD Biosciences).
Samples were analyzed on an Accuri cell analyzer.
All samples (50 000 events/sample) were analyzed on an LSRFortessa cell analyzer (BD Biosciences).
Cell-cycle profiles were obtained using a Fortessa cell analyzer (BD Biosciences) and produced using the FlowJo software (Tree Star).
Quality control measurements on the cells were recorded before the experiment using a bench cell analyzer sorter (Guava technology).
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