The extraction yields were evaluated by comparison of LC-ESI-MS/MS peak area ratios of OC/OCD3 in sample extracts against a calibrate solution of 1000 ng mL−1 of OC in 1 mL acetonitrile in water (1∶1), 0.1% formic acid.
The glass fibre laser Doppler probe (diameter 120 μm, wave length 810 nm, resulting penetration depth about 1 2 mm [ 13]) was calibrated using a calibration solution (Lawrenz GmbH, Sulzbach, Germany) and attached to a distal ileal segment with enbucrilate (Histoacryl®; Braun, Melsungen, Germany) without any compression or traction of the gut.
Drugs were added to the aCSF by means of a calibrated infusion pump from stock solutions 100 to 1,000 times the desired final concentrations.
The LDF device was calibrated with a standard calibration solution provided by the manufacturer.
Zn2+ standards of 0 1.5 p.p.m. were prepared from a 1000 p.p.m. calibrated stock solution in the same buffer as the protein sample.
Beginning on G6 and continuing daily until sacrifice on G16, the dams were weighed in the morning and provided with a wafer dosed with 0.5 μL/g body weight of a solution calibrated to deliver specific doses of PCB Mix 6 (Table 1).
The laser fluence profiles were experimentally measured through chicken breast tissue and calibrated intralipid solution at various imaging depths.
The final concentration of all amino acids present in the calibrated standard solution was 1.5 µM.
Prior to the procedure, the pH sensors were calibrated using solution buffered at pH 4.0 and 7.0, according to the manufacturer's instructions.
Do not use water in place of the pH 7 calibration solution to calibrate your pH meter.
Evaluation of matrix effects during LC-ESI-MS/MS was assessed by comparison of the OCD3 peak area in chromatograms of the calibrate solution and the sample extracts of the following degree of enrichment during SPE: 0, 100 100, 200, and 500 times.
