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Amplification was performed under the following conditions: 50°C for 2 min, 95°C for 10 min, and 40 cycles of 95°C for 15 s, and 60°C for 1 min. Transcript abundance, normalized to β-glucuronidase expression, was expressed as fold increase over a calibrated sample.
Experimental validation of the various systems was carried out by measuring the birefringent properties of a calibrated sample.
Relative quantification of microRNA expression was calculated as follows: raw data were presented as the relative quantity of target microRNA normalised with respect to RNU6B and relative to a calibrated sample.
The baseline correction was done using a calibrated sample of barium sulfate as reference.
The efficiency transfer method is considered to be a trendy model for calculating the full-energy peak efficiencies (FEPEs) of a sample of interest on the basis of an experimental efficiency curve measured in the same detector, but with a calibrated sample of a different size, geometry, density and composition [9].
Nevertheless, an analysis with a calibrated sample of respondents without response bias (n = 257) showed identical results to the main analysis regarding all factors of satisfaction (data not shown).
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We also used a low-volume pump (Sidekick, SKC Inc., Eighty Four, PA) to draw air at a calibrated sampling rate of 2.0 L per min for 10 h onto a 37-mm diameter, 0.3-μm pore size polytetrafluoroethylene membrane filters.
Uniform diffuse-scattering phantoms were imaged to measure and correct for the confocal and OCT depth scan response modulations in calibrated sample A-scans.
The 2 ml calibrated sample loop was overloaded to ensure it contained only the enzyme preparation to be desalted.
In order to present the relative changes in gene expression measured in qRT-PCR in the same scale as that found in microarray analysis the quantity of a transcript in one biological sample or state relative to that in a calibrating sample was expressed as log2 (fold change) in gene expression.
They are normally sampled by active sampling but recently a number of passive samplers that rely on calibrated sampling rates (Rs), have successfully captured the full suite of PAHs associated with both the gas and particulate phases.
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