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A calculated quantity of 1 mM silver perchlorate followed by 100 mM sodium borohydride and 3 mM of trisodium citrate was added to the above beaker under vigorous stirring.
0.5 %(w/v) of homogeneous gum stock solution was prepared by adding a calculated quantity of gum kondagogu powder into the reagent bottle containing milli-q water and stirring the same for 1 h at room temperature.
In short, at the first stage of the preparation of the polymerization mixture, a calculated quantity of the acid was added to the target PS latex portion and stirred at room temperature for 30 min.
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This information is fed into the bolus calculator, and the patient is presented with the calculated quantity of CHOs and the required insulin dose.
The quotient of the calculated quantity of substance to quantity introduced into the reactor gave a correlation coefficient which allows to give the actual value for the assumed value of concentration (1000 ppm).
In addition to chorus waves, various other waves are included in the observed waveform, which affects the accuracy of the calculated quantity of energy transfer.
At the third stage, the calculated quantity of the precooled to 10 °C KPS solution in distilled water was added dropwise into the reaction mixture followed by stirring for 24 h at 10 °C.
At the second stage, the calculated quantity of aniline was added to this acidified PS latex followed by stirring for 1 h to allow complete formation of the anilinium salt at room temperature and then the prepared mixture was cooled down to 10 °C for 30 min.
The calculated quantity of gp120 Env protein was approximately four to five times as high as the calculated quantity of p24 Gag protein.
Calculated quantity of the methanolic extract was dissolved in methanol in order to get 500 μg concentrations.
The calculated quantity of the target gene for each sample was divided by the average sample quantity of the housekeeping genes, glyceraldehydes-3-phosphate dehydrogenase (GAPDH) or 18S to obtain the relative gene expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com