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To study the mechanism by which tumour-specific modulation frequencies inhibit cell proliferation, we assessed the gene expression profile of HepG2 cells exposed to HCC-specific modulation frequencies using RNA-seq, as it provides a more comprehensive assessment of differential gene expression across a broader range of expression levels than microarray-based analysis (Wang et al, 2009).
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The top 100 most stably expressed genes with a broad range of expression levels were identified.
Our goal was to identify stably expressed genes with a broad range of expression levels from our Affymetrix data on the 526 samples (Table 1).
GLUT1 protein is expressed in most melanoma metastases with a broad range of expression levels as shown by Western immunoblot analysis [33].
By assessing the specific regions for mRNA folding, we could construct a predictive design method, UTR Designer, and demonstrate that proper codon optimization around the 5′-proximal coding sequence is necessary to achieve a broad range of expression levels.
The known porcine miRNAs have a broad range of expression levels, varying from millions of sequence counts for the most abundant to single count miRNAs.
However, we identified antibodies for 3 genes that show a broad range of expression levels, from the lowest quintile to the highest quintile: DnaJb1, DnaJb2, and Tomm70a (Figure 11).
Consistent with the results in Li et al. (2010) [ 21], porcine mature miRNAs had a broad range of expression levels.
A broad range of expression levels was observed but these were generally higher than in other normal tissues tested.
The identified genes have a broad range of expression levels, providing a variety of comparable and appropriate normalizing genes for any target gene of interest.
Purifications of N-S-β and C-S-β revealed ∼0.25−0.3 Y radical per β over a broad range of expression levels.
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