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Prior to Fourier transformation, free induction decays (FIDs) were multiplied by an exponential line broadening function of 10 Hz.
Use a broadened step function to renormalize the remainder of the QE(E) (i.e. for E > 626 eV).
Spectra were processed by NMRPipe with a 5 Hz line broadening function and analyzed by NMRDraw.
The discrete Fourier transform of a non-uniformly sampled sinusoidal pattern results in a broadened response function.
The validity of the fit was tested by fitting a Lorentzian peak prepared with a given set of parameters with eq 2 and by fitting the peak for CO obtained from spectra of the same sample but processed with different line broadening functions.
Taken together, these findings illustrate the fundamental relevance of miR-delivering by EVs for preserving physiologic conditions in the body and emphasize the relevance of further investigations in this field aiming at a broadened understanding of their functions and development of new personalized drugs in the future.
The formula assumes a Lorentzian broadening function and exactly ellipsoidal shape of the liposomes.
If there is no band overlap, neither NN nor PLS can calibrate the data accurately but the results could be improved by convolving the spectral features with a Gaussian broadening function.
Line widths of (5 GlcNAc resonances from the H C HSQC spectra, measured without applying a line-broadening function during processing, increased following attachment of the N-glycan to Fc and indicated structural differences between the different glycoforms.
The time domain signal was conditioned with a Gaussian line-broadening function equivalent to 50 Hz prior to Fourier transformation.
A 0.5-Hz line-broadening function was applied to all spectra prior to Fourier Transformation (FT) and baseline correction.
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