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In the model of control cells (condition 1), a branch segment was eliminated at 21 hours after the growing tip contacted other branches.
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A voltage stimulation is applied from the right end of branch segment "1" through a discontinuous initial action potential, which is given as follows: (21) V m (x, y ) = 25.4 mvolts if x > 3.5 − 84.6 mvolts otherwise.
Thus inferences about nodal values permit the estimation of evolutionary changes along each branch segment of an evolutionary tree [ 57, 58].
The radius of branch segment "5" is twice that of branch segment "6".
Branch segment "1" has the largest radius, equal to 160 μm and eight times that of branch segment "6".
The radius of branch segment "2" is six times that of "6".
In addition, branch segment length (the distance from one branch point to the next) for normal PC was constant at 5.5 ± 0.3 μm for all branches except the first branch segment (Fig. 4b).
We measured the branch angles and branch segment lengths of PC in 3-D space (Fig. 4a, b).
The radius of each branch segment takes one of the five values as the two-dimensional case.
However, in reeler PC, the branch segment length was significantly increased (Fig. S1b), and the number of branches was reduced (Fig. S1c), revealing a significantly reduced geometric complexity in the reeler PC.
In each of the simulations above, the action potential is initiated at the right end of branch segment "1" and propagates across the whole computational domain.
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