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This dataset is different from the enzyme dataset described in [ 19] in that all the structures include a bound native ligand.
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We also show that, in the absence of bound native substrates, these non-native ligands help define the critical 'hook points' in the DHAR enzyme active site.
The conformation of the bound native GlcNAc ligand was unambiguously determined based on the PUGNAc coordinates.
If ligand-bound native state of a protein were less stable than the ligand-free (apo) protein state, the ligand simply would not bind.
Presumably this is because the 35-305aa PAbindind more affectively to pvLDH 1-43aa-MAb-bound native pvLDH proteins than 1-43aa PAbs do, perhaps because of reduced competition.
In order to explore the effects of added GDP on agonist binding, we reduced membrane-bound native GDP by inducing its dissociation under slightly denaturing conditions, washing and renaturing as described in Methods.
The spore appears to present a bound antigen in its native conformation promoting a cellular (Th1-biased) response coupled with a strong antibody response.
Curiously then, as Sahlins shows, Obeyesekere turns eighteenth-century Hawaiians into twentieth-century modern Europeans, living up to the highest Western standards of "practical rationality". By contrast, Western scholars are turned into classic custom-bound "natives", endlessly repeating their ancestral traditions of the White man's superiority by insisting Cook was taken for a god.
DNA with a bound tetraintercalator displays a retarded gel mobility so that bound and unbound radiolabeled DNA can be quantified using native polyacrylamide gel electrophoresis (PAGE) and autoradiography.
A native gel-based assay was then used to monitor unwinding by the increase in fluorescence in a closed-state core containing a bound single strand.
A bound Wizard will spawn.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com