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(Left) Spike rasters showing discharge in response to successive 3.5-ms light pulses for a grid cell and a border cell.
The firing of a single border cell recorded in a late session also replicated between the two compartments.
The ETS transcription factor Aop also plays a role in border cell migration and does not receive input exclusively from EGF, but from a number of signalling pathways including Notch [ 95].
par-6, a known regulator of border cell migration, was a positive hit and thus validated the approach.
The proposed models are referred to as a buffer cells model (BCM) and an external border cells model (EBCM).
To address whether Bbg regulates a specific aspect of border cell migration, we analyzed the levels and localization of several border cell-enriched proteins.
We also observed that many genes which encode cell wall modification enzymes, such as cellulose synthase and cell expansin in the lateral root region of Vagad, are considered as playing a key role in border cell separation and the elongation of the root [ 55- 57].
During Drosophila oogenesis, the so-called border cells perform a stereotypic migration between germ cells toward the oocyte to form the future sperm-entry point., As a simple and genetically tractable in vivo model, border cell migration provides a good platform for our understanding of collective cell movement.
We previously showed that upstream Hippo pathway components have a key role in promoting border cell migration by regulating the actin cytoskeleton (Lucas et al, 2013).
The advantage of using RNAi to test a selected class of genes, like the one performed here, is the rapid identification of genes involved in a particular process such as border cell migration.
bbg RNAi border cell clusters contain a similar number of cells compared with control clusters.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com