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To induce CIA, male Sprague Dawley rats were immunized with bovine type II collagen emulsified in Freund's incomplete adjuvant, followed by a booster injection 14 days later.
At 35 days of age, pigs on both diets (2 × 2 factorial design) received either an injection containing hen eggwhite lysozyme (HEWL), killed Mycobacterium tuberculosis and Freund's complete adjuvant (immunized) or phosphate buffered saline (PBS) (non-immunized) into the neck followed by a booster injection 2 weeks later and induction of delayed-type hypersensitivity (DTH) one week later.
A booster injection was given on day 20 that is nearly three weeks after the first injection.
On day 21, a booster injection of 100 μg collagen in incomplete Freund's adjuvant was given at the base of the tail.
Mice were immunized with 50 µl of OVA257-264-liposome and 2, 4, 8, 16, and 20 weeks later, the mice received a booster injection with OVA.
As shown in Figure 3, a complete cell killing was observed at 2 weeks after the immunization without a booster injection and, up to 20 weeks after the immunization, a significant recall response was observed upon booster injection with OVA.
This was repeated as a booster injection 21 days later.
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We repeated the experiment using a reduced collagen induction schedule, featuring a reduced booster injection (reduction to 80%, 0.04 ml emulsion instead of 0.05 ml).
These recommendations state that, for patients with major or contaminated wounds who have had a tetanus vaccine 5 10 y previously, a single booster injection of tetanus toxoid must be promptly administered on the day of injury., Both Tdap-IPV and TMV were generally well-tolerated and participants in both groups experienced a similar incidence of injection-site reactions.
18 days later mice received a first booster injection of 20 µg antigen and a second booster 24 h before the hybridization on day 82 of the immunization process.
A second booster injection with 10 U/0.1 ml dose, was given within a 10-day interval to those HDPs responding with a <10 mm induration to the first test.
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