In this case, a binding line was drawn linking the second and third hits to the best hit of the same Citrus query.
The STRING network contained 16 proteins/genes, and 45 gene pairs connected by 47 links, including five different types: activation (four links), expression with inhibition (seven links), binding (one link), post-translational modification (one link), and functional links (34 links).
We added relevant "binding" links and specified references where a protein/microtubules or protein/protein association was previously described.
Comparable kings who also embodied theories of divinely-ordained rule, rightful sovereignty and the binding links between king, land and society, include: Conaire Mór, Cormac mac Cuilennáin, King Dómaldi and Halfdan the Black.
Since protein shedding will only occur when the highest (electrostatic) affinity link is cleaved, the other binding links should simply not be detected and the resulting map will always reflect the strongest associations within the complex.
We have established a new protein-engineering strategy termed "directed domain-interface evolution" that generates a binding site by linking two protein domains and then optimizing the interface between them.
Therefore, this region may represent a binding surface that links Shroom proteins to regulators or effectors and likely plays an important role in the spatial regulation of Shroom function.
The N terminus of the molecule (the heavy black track in Fig. 1A) includes the link module (92 residues), which shows 35% homology to other HA-binding link proteins.
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We note because most location analysis programs link a binding site with the nearest transcription start site, we had to modify our location analysis program to allow appropriate characterization of ZNF274 binding sites.
