Sentence examples for a best alignment to from inspiring English sources
Twenty-six (54%) of the non-conserved miRNAs had a best alignment to Arabidopsis sequences, of which two predicted target genes were annotated only as expressed sequences.
Bismark fully bisulfite converts sequence reads and maps each to bisulfite converted versions of the reference genome, with only reads producing a unique best alignment to a region being kept.
Using an informatics pipeline that allowed up to two mismatches in a read to map to the reference, we found that 43.9% of the B73 fragment reads had a single best alignment to the reference, 46.7% could be aligned to multiple positions, and 9.3% could not be aligned to the reference at all.
Cleaned reads were mapped to the maize B73 version 2 pseudomolecules (AGPv2; http://ftp.maizesequence.org/) (Schnable et al. 2009), using Bowtie version 0.12.7 (Langmead et al. 2009), allowing up to two mismatches and requiring a single best alignment to nuclear DNA.
The best alignment to a sequenced Micromonospora CBM family 33 protein (NCBI reference sequence WP_007071991.1) resulted in 70% identity.
This requirement forces the best alignment to match a majority of the probe sequence, since BLAT produces many spurious short alignments with 100%percentt identity.
As earlier common computational methods for identifying precursor genes, a gene with the best alignments to a chimeric mRNA was considered as the precursor gene [ 7].
With this new block, the task is to search for a best alignment of the input sequences allowing the use of gaps, matches, mismatches and differences.
The assignment of assembled sequences to chromosomes was done based on a best alignment and majority rule.
Likewise, the score of a best alignment ending in an unconserved (intergenic) region cannot be calculated from the score of a best alignment ending in a conserved region (intron) of the two sequences.
Given this fact, the score of a best alignment ending in a conserved (intronic) region cannot be calculated from the score of a best alignment ending in an unconserved (intergenic) region of the input genomic sequences.
