The phrase "a bead next" is not correct and does not convey a clear meaning in written English.
It may be intended to describe the placement of a bead in relation to something else, but without additional context, it is unclear when to use it.
Example: "I placed a bead next to the other materials for the project."
Alternatives: "a bead beside" or "a bead adjacent to".
Tissues (15 50 mg) were homogenized 1 10 in isolation buffer (100 m m KCl, 40 m m Tris HCl, 10 m m Tris Base, 5 m m MgCl2, 1 m m EDTA, 1 m m ATP, pH = 7.5) with phosphatase and protease inhibitors (HALT, Thermo Scientific, Rockford, IL, USA) using a bead homogenizer (Next Advance Inc., Averill Park, NY, USA).
Nonspecific binding was precipitated with pre-swollen protein A-Agarose beads (P3476, Sigma).
The Gene Ontology gene sets had the lowest average F-scores compared to the genomic and gene family subnetworks (F RNAseq =0.044, F Bead =0.029, F Oligo =0.039).
Endogenous HSPA8 was immunoprecipitated using Sepharose Protein A beads (Sigma #P3391) to which the antigen-anti-HSPA8 antibody complexes were bound, according to the manufacturer's protocol with modified centrifuge speeds which were kept below 4,000 Xg.
For lipid extraction of ear tissue, 29.8 mg in methanol (29.8 mg tissue/mL) were pulverized and homogenized in a bead pulverizing machine (6500 rpm for 15 s, ×2), 200 μL of homogenized solvent were transferred to a 2-ml glass tube, 100 μL of chloroform were added, and the tubes were vortexed for 10 s.
Following incubation with DNA templates and biotinylated ePCR primers as described above, the beads were resuspended in a mixture of 1X Pfu buffer, 0.2 mM each dNTP, 0.1 µM TR homoprimer, 0.1% Tween 20, and 0.2 U/µL Pfu Turbo polymerase (Stratagene, La Jolla, CA) at a bead concentration of 5×104/µL.
A four hour incubation of previously isolated liver RNA with RNase-free beads or untreated beads (Next Advance) demonstrated that RNA is not degraded when in contact with the untreated beads.
Although New Yorkers have obsessed, haggled and boasted about Manhattan real estate since its controversial and possibly apocryphal exchange for a handful of beads four centuries ago, it is fair to say that the promotion of Manhattan apartments has exploded over the past few decades.
The Studio Museum in Harlem will present a bead-making workshop tomorrow at 10 30 a.m, for children 6 and older who will create "story bracelets," at 144 West 125th Street, (212) 864-4500, extension 230.
The Studio Museum in Harlem will present a bead-making workshop tomorrow from 10 30 a.m. to 12 30 p.m. for children 8 and older and their families, at 144 West 125th Street, (212) 864-4500, extension 230.
