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Attach a bead for the clasp hole to fit over.
Draw a doorknob on it, or glue on a small lid from a tube or glue on a bead, for the door handle.
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Total protein lysate (500 μg) was incubated with 30 μl of the protein A beads for an hour at 4°C and centrifuged.
Add a button or bead for the mouth.
To overcome this limitation, Yasuda's team substituted a 40-nanometer-diameter gold bead for the clumsy filament.
Add a necklace and bracelets made of wooden bead for the final touch!!
Cleared lysate was immunoprecipitated with the appropriate antibody, prebound to Protein A Sepharose beads, for the indicated time at 4°C.
Note that some tire irons have a flat pointed end that works better for breaking a bead than the spooned end of the iron.
After you got an even numbers beads for the necklace, adjust the bead at center position to make whole into two lines; after that, weave the stain ribbon between the two beads lines.
A simple protein extraction protocol was established, using a bead mill for homogenizing fly specimens and the denaturing conditions of 6 M urea for efficient solubilization of proteins.
Then, you can add a piece of bent wire as a faucet, and red and blue beads for the hot/cold switches.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com