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Attach a bead for the clasp hole to fit over.
Draw a doorknob on it, or glue on a small lid from a tube or glue on a bead, for the door handle.
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We immunoprecipitated 1 mg of protein samples in a total volume of 1 ml with 2 μg of antibody and 20 μl of Protein-A beads (for rabbit polyclonal antibodies) or Protein-G beads (for mouse monoclonal antibodies).
Lysates were pre-cleared with protein-A sepharose beads (GE Healthcare), incubated with antibodies (2.5 to 5 μg) for 2 hours to overnight at 4°C with rotation, and then with protein-A beads for 1 hour at 4°C with rotation.
This study investigated potentials of fluidized bed systems with zeolite A beads for removal of heavy metal ions from aqueous solutions.
The maximal sorption capacity of zeolite A beads for Cu II) at 20 °C was predicted as 23.3 mg g−1 based on the Langmuir model applied to the sorption isotherm.
Lysates were precleared for 2 h at 4°C with protein A-sepharose (Amersham Biosciences) then incubated with mAbs pre-adsorbed onto rabbit anti-mouse-protein A beads for 2 h at 4°C.
Extracts were incubated with mouse anti-c-myc (a kind gift from T. Ravid) overnight and with anti-Protein A beads for 2 hours before extraction into 2× sample buffer.
Samples were then incubated with 40 μL of protein A beads for 2 h.
Anti-caspase-8, anti-IRE1, and anti-RIPK1 antibodies were coupled to protein A beads for immunoprecipitation.
Total protein lysate (500 μg) was incubated with 30 μl of the protein A beads for an hour at 4°C and centrifuged.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com