The phrase "a basta" is not correct or usable in written English.
It appears to be a mix of languages and does not convey a clear meaning in English.
Example: "After all the delays, I told them, 'Enough is enough, a basta!'"
Alternatives: "That's enough" or "Stop it."
Raphael calls the third stage in Italian manner as a basta stage, when you can research for a long time, see the general picture or get down in the weeds and lost in the particulars.
Now that the weather's finally turned in time for the holiday weekend, drop by Everything But Water's first New York shop for a selection of specialty swimwear; a Basta Surf strappy bandeau top ($106) and reversible hipster bottoms ($99) look well suited for that first StndAIR trip.
For our constructs, we exchanged the hygromycin resistance marker for a Basta resistance marker.
POLAND: J. Kornafel, M Litwiniuk, H Karnicka, L Bablok, Marianowski, A Basta and A Jakimiuk.
The first vector, named pB35GWG, contains a BASTA resistance gene for selecting transgenic plants and a C-terminal GFP tag.
The insert of a correct clone was cut out with NotI and cloned into the plant transformation vector pBART [ 38] which is identical to pART27 [ 39] but contains a basta- instead of kanamycin-resistance gene for plant selection.
pCASP1 NLS-GFP-GUSS and pCASP1 NLS-GFP-GUS repCASP1 NLS-GFP-GUS into andasta-resistance pCASP4II vector and contain 1048 bp and 695 bp promoter fragments, respectively.
pSGN3 (5583 bp before ATG) ::SGN3 gDNA-mVenus was cloned by integrating a 9.4-kb genomic fragment including the intron and the 5′UTR into a Basta-resistance pGREENII vector containing mVenus.
Among the pCAMBIA-derived binary vectors, those with a hygromycin-resistance gene as a selectable marker were derived from pCAMBIA1300, while those with a kanamycin-resistance gene were derived from pCAMBIA2300, and those with a Basta-resistance gene were derived from pCAMBIA3300.
For example, comparative sequence modeling predicts that all RV-C VP1 proteins are shorter by 21 residues compared to VP1 proteins from RV-A strains (Basta et al., 2014).
Lazio lost 2-1 Atalantantasas a Dusan Basta own goal and an Alejandro Gómez effort overcame Lucas Biglia's opener, while Milan beat Chievo with Luca Antonelli scoring the only goal of the game.
