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A background spectrum of 10 mM ammonium acetate was subtracted from all αSyn spectra.
Before each sample measurement, a background spectrum of a gold-coated substrate was recorded with a spectral resolution of 4 cm–1 with 30 co-added scans.
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This could suggest that the West of Scotland population has a wider background spectrum of deprivation or that the deprivation gap is widening on a national level over time.
A background spectrum was acquired on a clean part of the ZnSe slide and 40 to 50 spectra of each yeast sample spot were recorded at different positions.
For each sample, a background spectrum was generated as the average of five spectra acquired from the liquid around the cell.
It has been found that the background spectrum of a HPGe detector can be scaled down with the shielding depth.
Upon reduction, detailed features in the background spectrum of unreduced samples, which may be assigned to surface or multiphonon modes, vanish while weak bands due to electronic transitions appear.
Samples were scanned from 4000 to 400 cm−1 (2500 to 25,000 nm) at 4 cm−1 resolution and corrected against the background spectrum of the gold standard.
Compared with the background spectrum of the Ag/GL-1/0.5-25, the intensities of two Raman bands centered at 1046 ± 8 cm-1 and 688 ± 3 cm-1 are much higher, while the other bands diminished or even disappeared.
The background spectrum of buffer without protein was subtracted from the protein spectra.
The background spectrum of pure KBr was subtracted from the spectrum of the sample.
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