Sentence examples for a backbone for generating from inspiring English sources

This vector served as a backbone for generating six silent mutations in the LAMR coding sequence using the Quick change II XL site-directed Mutagenesis kit (Stratagene, La Jolla, CA, USA).

Considering the endemicity that clade 2.3.4 subtype H5N1 viruses have gained in China since 2005 (7 – 9 ), it is plausible that subtype H5N1 viruses have provided the backbone for generating the novel subtype H5N5 viruses instead of the opposite gene flow.

We used Comparative Toxicogenomics Database (CTD, http://ctdbase.org/) as a database for generating a backbone multi-type interaction network[ 13].

The sleeping pictures acted as sort of a backbone for the project.

Twitter is a dream for generating leads.

The pUC18 plasmid was utilised as the backbone for all generated constructs.

Recommendation #6: GROW A BACKBONE.

Results indicate that game theory based strategies and associated constraints provide a determinative backbone for an efficient and effective use of generated Pareto Frontier Surfaces.

To generate an inducible TEV protease, a codon-optimized protease sequence containing two artificial introns was synthesized as two gBlocks (IDT, Coralville, IA) and assembled with the hsp-16.41 hsp-16.41and unc-54 3ʹUTR to generate promotery digesting pWD141 (M Wande Davis, University of Utah) wito BstBI and EcoRV to generate a vector backbone for the Gibson reaction.

Subsequently, the shRNA sequence mediating the most effective knockdown was cloned into a lentiviral vector backbone to generate viral particles.

From the pRSFDuet-1/sgRNA expression vector, a 4,221 bp-long amplicon (expression vector backbone) was generated by PCR (1× 98°CC for 2 min; 30× 98°CC for 10 s, 57°C for 30 s, 72°C for 4 min 12 s; 1× 72°CC for 5 min) using two uracil-containing primers (sgRNA Backbone_fw and sgRNA Backbone_rv, Integrated DNA Technologies, Supplementary Table SII) and the X7 DNA polymerase (Nørholm, 2010).