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We then asked if HA-CENP-A (a mark for newly assembled CENP-A) assembled on the HAC and endogenous centromeres at 24 h (i.e. one complete cell cycle in HeLa cells) after transfection.
CENP-A assembled on ectopic alphoidtetO arrays was maintained in metaphase cells, where the ectopic HA-CENP-A was always detected as an extended region weakly stained with DAPI.
This result implies that CENP-A is assembled on a similar proportion of DXZ1 HOR alpha satellite irrespective of total array size.
It showed a handful of German soldiers with the population of a Russian village assembled on one side of a bridge, divided into men and women.
At midday, Washington took his oath standing on a balcony above a crowd assembled on Wall Street.
On a nearly cloudless day in late January, Newt Gingrich stood in front of the P.G.A. Museum of Golf, in Port St. Lucie, Florida, and addressed a modest crowd assembled on a putting green.
Shedd's plight shows the potential consequences for unsuspecting homeowners, who can become innocent victims of a housing market assembled on a mountain of fraud.
He eventually ends up lecturing to an assembled crowd on a bus.
All images were acquired on either an N-STORM microscope (Nikon) or a custom-built setup assembled on a commercial Axio Observer D1 microscope stand (Carl Zeiss).
"They beheld a group of people assembled on a street corner," wrote Thomas C Romney, their grandson, in 1948.
The size of a single migration chip assembled on a cover slide was 2×3 cm.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com