Exact(17)
For F. verticillioides (anamorph stage of Gibberella fujikuroi, mating population A) assays, nine seeds of each GM event, plus Senia and S-hgr lines, were surface sterilized and incubated for 30 min in the presence of a suspension of F. verticillioides conidia (10 spores/ml) under agitation.
The antisera were sequentially absorbed on various human non-tumour cells, reactivity being monitored by immunofluorescence and 125I-labelled staphylococcal protein A assays.
The widespread prevalence of the 017 group of A-B+ strains in Asian countries shows that assays for toxin B or the tcdB genes are preferable to toxin A assays for diagnosis of CDI.
Parallel transfection of wild-type GLA was considered to be the most appropriate transfection control for missense and small deletion or insertion GLA mutants, as the cDNA sequence differs only by one or a few base pairs, and it is the most commonly used transfection control for transiently expressed mutant α-Gal A assays [Fan and Ishii, 2003; Ishii et al., 2007].
All IL-1β 86/680 measurements were lower than nominal values with the notable exceptions of in-house Luminex (laboratory I) and Beadlyte Luminex (laboratory A) assays.
Approximately 25% of Track A assays and 39% of Track B assays had a low potential for mispriming, indicating that the likely conversion rate for PRTs generated on masked sequence will be higher than unmasked sequence.
Similar(43)
The TGF- β1, M-CSF, and VEGF-A assays detect specifically the biologic active form of the protein.
Detection limits were 0.7 pg ml−1 and 9 pg ml−1 for the IL-6 and VEGF-A assays, respectively.
Cystatin-C, VCAM-1, and Pappalysin-A were quantified using the respective Human Cystatin-C, Human sVCAM-1, and Human Pappalysin-A assays (R&D Systems, Abington, UK) according to the manufacturer's protocols.
Next, the collaborating laboratories used the PIGRET and RBC Pig-a assays to assess the mutagenicity of a total of 24 chemicals in rats, using a single treatment design and mutant analysis at 1, 2, and 4 weeks after the treatment.
First, the technical proficiency of the laboratories and transferability of the assay were confirmed by performing both the PIGRET and RBC Pig-a assays on rats treated with single doses of N-nitroso-N-ethylurea.
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