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Here we describe a botulinum neurotoxin serotype A assay with a large immuno-sorbent surface area (BoNT/A ALISSA) that captures a low number of toxin molecules and measures their intrinsic metalloprotease activity with a fluorogenic substrate.
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The organic phase was measured at wavelength of 280 nm for MAO-A assay with spectrophotometer, respectively.
With respect to serum troponin assays, a CV of <10% is considered ideal, and an assay with a CV of >20% should not be utilized, in general.
It should also be noted that that we defined a positive HCV RNA result on the basis of an assay with a detection limit of 2500 copies/ml.
Earlier studies used a quantitative assay with a limited functional assay sensitivity of 0.3 to 0.5 μg/L, whereas more recently an assay with a functional sensitivity of 0.06 μg/L has been available [ 28].
For an assay with a normal distribution this would equal mean plus (just under) one standard deviation.
This was therefore investigated further in an assay with a much larger basal to maximum window (see below) where these smaller details are more easily seen.
It would be advantageous to develop an assay with a protein that is associated with the development of bladder cancer.
In an assay with azocasein as a substrate (azocasein assay), the purified Sso0660 protein showed a specific activity of 7.2 units·mg−1·h−1.
The best inhibitor showed an IC50 = 10 μM in an assay with COS7-cell lysate overexpressing murine ATGL.
We have developed an assay with the capacity to infer chromatin structure on a genome level in living organisms.
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CEO of Professional Science Editing for Scientists @ prosciediting.com