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Data were also analyzed using combined endpoints A (ARF, RVAD) and B (IC, ARF, RVAD).
Higher levels were seen throughout the study, but particularly at recruitment, in group A (ARF) than in group B. Concentrations of C-reactive protein were elevated in all patients on recruitment, and remained elevated throughout the study in all except two patients, both with preserved renal function.
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At low auxin concentrations, a heterodimer of an ARF and an Aux/IAA protein represses transcription.
To analyze if HRG had a direct effect on glial cell proliferation, primary glial stem cell cultures were set up from neonatal Ntv-a Arf-/ mouse brains.
We used the Ntv-a Arf-/ mice and induced tumors with RCAS-PDGF-B, a combination that mainly produces high-grade gliomas [27] and analyzed how simultaneous expression of HRG would affect brain tumor development.
We have investigated the effect of the antiangiogenic protein HRG on orthotopic PDGF-B induced glioma development in vivo using the Ntv-a Arf-/ mice that generate a high proportion of malignant tumors.
Primary glial stem cell cultures were established from neonatal Ntv-a Arf-/ mice and cultured in N2 medium on polyornithine/fibronectin coated tissue culture plates with addition of 10 ng/ml FGF-2, as previously described [30].
To analyze the effect of HRG on glioma development neonatal Ntv-a Arf-/ mice were intracranially injected with equal numbers of DF-1 RCAS-PDGF-B-eGFP [33] and DF-1 RCAS-HRG or DF-1 RCAS-X (control virus, complete RCAS without inserted exogenous gene).
INK4 a-ARF deletion analysis was performed as recently described for the characterisation of mono- and biallelic 9p21 deletions in childhood acute lymphoblastic leukaemia (Bertin et al, 2003).
The set_arf command assigns an ARF to a dataset.
b. c The simulated line profiles when using an ARF with a scattering kernel, 5 and 10 cm, respectively, from the collimator surface.
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