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The first ("A") aliquot was digested with McrBC, which cleaves methylated DNA sequences, leaving unmethylated DNA intact.
After drying of [18F]F-, aliquotof of DMSO was added to dry [18F]F-/K222/K2CO3 compriorprior to the addition of [18F]FTP precursor during the manual radiolabeling.
A aliquot (200 µl) of this sample was resolved on a Superose 6 HR 10/300 gel filtration column (GE Healthcare) in the same buffer.
The pre-amplified cDNA was diluted 1∶4 in 0.1X TE buffer (pH 8.0), a aliquot of which was further diluted 1∶50 and 1 µL was used in a 5 µL qPCR using standard conditions to profile 312 mouse miRNAs and the 6 reference genes (single replicate).
A aliquot (10 μg protein) of each lysate was subjected to 10% SDS-PAGE, and the separated proteins were electrophoretically transferred to nitrocellulose membranes.
After centrifugation for 10 min at 1,800 g, the pellet and fluffy layer were collected and resuspended in 80 ml of medium A (Aliquot S2).
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I cannot eat more than two or three ounces at a time, but an aliquot of gefilte fish every waking hour nourishes me with much needed protein.
Then, an aliquot of a 1% sodium citrate aqueous solution is added.
The quality of RNA was verified by running an aliquot on a 1% agarose gel.
The PCR enriched libraries were validated by running an aliquot on a standard agarose gel.
At a preidentified time interval, an aliquot was removed and replenished with fresh medium.
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