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Similar to those observed in other species, nucleotides most frequently added to zebra finch miRNAs were U and A, with the U additions occurring more frequently than the A additions (66 miRNAs had U additions and 35 miRNAs had A additions, Additional file 10).
To estimate the length of poly(A) additions, poly(A) tracts were removed by RNase H digestion in the presence of oligo dT) (Hausen and Stein, 1970).
It has previously been reported that on its own in vitro, mtPAP was unable to extend 3′ termini beyond short oligo(A) additions and required the presence of LRPPRC to promote extension (25).
Finally, we reveal that the kinetic preference for catalyzing the removal of pyrophosphate from U 1 A73-containing tRNAHis effectively limits the ability of SceThg1 to efficiently add sU 1 A73-containingdes tRNAHistrateffectivelyminate with a U:A base pair, thus rationalimits the previously observed labilitymultiple U of A additionSceThg1e 3′–5′ polymerase actovity (Tablefficiently
Similar(54)
A-addition was performed (Qiagen A-Addition Kit 231994).
It's an addition, an enrichment, an enhancement.
It's an addition, not a substitute.
We always said it is an addition not a replacement.
This is a gem of an addition.
"Infrastructure" is an addition, and possibly a diversion.
We put on an addition; we put in a pool.
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CEO of Professional Science Editing for Scientists @ prosciediting.com