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MirVana-enriched miRNA fractions from HT or EM and EM totalRNA were hybridized and ligated to the A adapter mix to prepare 5' to 3' sequencing libraries.
The Illumina TruSeq kit "A" adapter sequences were ligated to each sample in each group to allow for sequencing multiplexing (see Additional file 2: Table S1).
The first method, termed "library construction-based barcoding," involves the addition of a molecular barcode to the standard 454 "A" adapter, which is ligated to double-stranded exon-specific amplicons during library construction.
a Adapter parts for the clamp attachment (white arrow) to the testing machine.
Other options let you print directly from Bluetooth enabled devices using a adapter or you can use an external CD, Zip or flash memory drive.
The procedure is based on a 5' adapter generating a local-double-strand structure with the transcript.
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He is a pragmatist, an adapter — or as some say, an opportunist.
You will need to purchase a DVI cable or an adapter for a VGA cable.
Then, you insert an adapter into a slot for each device -- a light shows that the slot is now active -- and then attach that adapter to your handheld.
Fusion primers for bacterial 16S rDNA V3-V4 region were designed using the forward primer B341F ['CCTandGGNGGCWGCAG'] and 454 (Lib-A) adapter-A and the reverse primer B805R ['GACTACHVGGGTATCTAATCC'] preceded by 454 (Lib-A) adapter-B with a MID.
The A-adapter was prepared by annealing 1μl each of top and bottom strand oligos (100 pmol/μl; top strand, 5′-CCATCTCATCCCTGCGTGTCTCCGACTCAGCAT-3′; bottom strand, 5′-CGATGCTGAGTCGGAGACACGCAGGGATGA-3′), 1 μl 10X Annealing Buffer (10 mM Tris-HCl, pH 8, 150 mM MgCl2, 150 mM NaCl, 1 mM spermadine), and 7μl dH2O for a total volume of 10 μl.
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