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Potential failures are (a) accidental alignment between two adjacent nuclei that form a partial fold with insufficient information for partitioning them (shown in Supplementary Section S5) and (b) sequential RANSAC inferring incorrect partitioning because of its greedy nature; however, multi-RANSAC (Isack and Boykov, 2012) should reduce the frequency of incorrect plane fitting.
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This problem is more significant in the Z direction because of its lower resolution and potential accidental alignment of two overlapping nuclei along the Z direction.
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