Sentence examples similar to With transfection complete from inspiring English sources

Twenty-four hours after transfection, complete medium supplemented with puromycin (10 μg ml−1) was added and cells were further incubated for 72h.

Six hours after second transfection complete medium with or without 0.1 mM pantethine was added.

In the experiments where estrogen (Sigma-Aldrich, St . Louis MO, USA) was added, the complete growth medium with transfection reagent was removed; cells were washed and replaced with serum-free medium containing 100 n m estrogen for further 24 h.

Complete growth medium was replaced with transfection medium 24 h prior to loading.

Forty-eight hours after transfection, the complete medium was removed and replaced with serum-free Opti-Mem for subsequent plasmid transfection.

Before transfection, the complete medium was removed and cells were rinsed once with 1x PBS.

After 6 h of transfection, DMEM complete medium was added.

Culture media were changed 8 h after transfection for complete DMEM media, in order to remove transfection reagents and avoid possible contamination of the incubation media with untransfected oligonucleotides.

The silencing effect lasted up to 9 days following transfection with nearly complete suppression shown at least six days after transfection.

Cells were infected or mock-infected 16 h post-transfection or simultaneously with transfection (live-cell imaging) at indicated MOI.

All of these genes demonstrated growth-suppressive properties with transfection into melanoma-derived cell lines.