Sentence examples for With dideoxy sequencing from inspiring English sources

We confirmed every variant call with dideoxy sequencing to determine the false positive rate.

The algorithm settings were determined after evaluating different quality score thresholds and comparing array calls with dideoxy sequencing results to characterize coverage and accuracy.

We also calculated the number of exons per array that would need to be interrogated with dideoxy sequencing in order to resolve no-calls and confirm positive calls.

Overall, caution is warranted when performing validation trials with dideoxy-sequencing as the golden standard to determine the specificity of methods with higher mutation detection efficiency on FFPE-DNA templates.

Tumor FFPE-DNA from 135 diagnostic and 75 low-quality control samples was obtained upon macrodissection, tested for fragmentation and assessed for KRAS mutations with dideoxy-sequencing and with two Q-PCR methods (Taqman-minor-groove-binder [TMGB] probes and DxS-KRAS-IVD).

Instead, these studies all used the v3.4 assembly, which is publicly available since 2004 [ 13] and contains many gaps, assembly inconsistencies and nucleotide and indel errors (due to the relatively low coverage and typical errors associated with capillary dideoxy sequencing).

Resequencing array-based mutation detection has been reported to produce a throughput of nearly 100 patients per technician per month and can thus be used as a method for initial genetic screening while being supplemented with conventional dideoxy sequencing for samples in which the array cannot identify a causative mutation [ 13].

Tail swap mutants were constructed with triple cloning procedures, then the junction sites were confirmed by sequencing both strands with dideoxy DNA sequencing using appropriate primers and a Sequenase version 2.0 DNA sequencing kit (Amersham).

Dideoxy sequencing with T7 primer for pcDNA3.1 or with a specific primer for pCM185 was conducted using the T7 Sequencing kit (Amersham Biosciences), for individual constructs expressing rTFs.

Mutated TSHR sequences were verified by dideoxy sequencing with dRhodamine Terminator Cycle Sequencing chemistry (ABI Advanced Biotechnologies, Inc., Columbia, MD).

Conflicting results were resolved using Sanger dideoxy sequencing with BigDye terminator chemistry (Applied Biosystems, Foster City, CA) and an ABI 3700 Analyzer instrument (Applied Biosystems).