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After another 24 h, cells were washed with PBS and detached with trypsin.
Cells were first washed with PBS and detached with 0.2 mL of 0.25% trypsin.
Cells were then rinsed with cold PBS, detached with tripsin and centrifuged.
The cells were then washed with PBS and detached with trypsin at 37°C.
Infected cells were washed with PBS, detached using Cellstripper (Cellgro) and neutralized with DMEM +10% FCS.
At 20 °C, the cultured NIH/3T3 cell sheet with bFGF detached from heparin-functionalized thermoresponsive surface.
Afterwards, cells were washed with PBS, detached and fixed in 70% ethanol (24 h, -20°C).
Metal rod with chain, hand with pointer finger (detached); used for reading scripture.
The cells were detached with trypsin, washed with PBS, and fixed in 2% paraformaldehyde.
Cells were washed with phosphate-buffered saline (PBS) and detached with 0.1%.
Then, cells were detached with trypsin/EDTA, washed with PBS and frozen in liquid nitrogen.
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