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The presented validation exercise demonstrated that the extended bristle friction model can well duplicate experimental results including typical frictional behaviors such as sliding, sticking and stick-slip.
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Numerical simulations using finite element method well duplicated the elasticity and plasticity of this material.
Then, a t-test was performed, and three well duplicates were selected to identify differentially expressed genes.
The lysate (10 µL) was added to two wells (duplicate) of the 96-well plate and mixed.
Each group was analyzed in two wells (duplicate) and the experiment repeated three times with cells from three different animals.
In this section we extend the distance measure to include delete operations as well as duplicate and duplicate-invert operations.
All plates contained at least two-well duplicates for each primer and cDNA condition.
Samples (100 µl per well in duplicate) were loaded in ELISA microtiter plates (Immunoplate, Maxisorp, Nunc) coated with capture antibodies.
Test solutions were added at 25 μl to each well in duplicate for each experiment.
With this approach, however, a few well described duplicate clusters are not identified.
After washing as above, standards and samples were applied at 25 µl per well in duplicate.
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