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We streak like superheroes past suns and solar systems, we dive through shoals of quarks and atomic nuclei.
How many times in a lifetime do we streak off in a direction contrary to the call we hear in our heart, only to find ourselves tempest-tossed, downcast, and at the point of despair, looking prayerfully upward from an abject situation, resolving to do better if only given another shot, and in the end wind up giving thanks for the tremendous miracle of a second chance!
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To test whether the "military" and in vitro passaged strains possessed low-grade hemolytic activity, we streaked these variants on rich agar media containing 5% sheep's blood and looked for hemolysis.
To obtain clonal isolates, we streaked out aliquots of frozen samples (-80°) harvested from the evolving populations at regular intervals on PYE agar plates and isolated individual colonies.
We streaked each of the 115 clones from the E. coli thermal stress experiment from frozen glycerol stocks onto tetrazolium and arabinose (TA) agar plates and grew them for one day at 37.0 °C.
When we streaked out an E. coli colony containing S0524M13 that appeared to contain all four exons of the sdY gene and tested individual BAC clone colonies from this progenitor by PCR using combinations of primers from exons 1, 2, 3, and 4 (Table S1 and Figure S1), we found that often exon 1 and occasionally exon 2 were missing.
DOI: http://dx.doi.org/10.7554/eLife.04629.017 To test whether the rate of FA synthesis is also important for C. glutamicum L-form proliferation, we streaked growing walled and L-form cells on NA/MSM plates in the presence of 2 μg/ml of cerulenin.
To measure effects on cell migration we used streak assays, where the ability of cells to repopulate a wounded area in a monolayer can be measured.
"I wanted to keep believing that we had a streak because we hadn't had one all year," Manager Bobby Valentine said.
"We had a streak coming in and we believed coming in we were the better team," Josh Boone, a Connecticut freshman forward, said.
"When we are asked, we remember that we are on a great streak, but we really don't care.," Ginobili said Monday after practice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com