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We observed affected localization of NPC-associated proteins, diminished lamina dynamics for both LaA mutants and reduced nuclear import of representative cargo molecules.
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The chemical shift changes of Ubiquitin that we observed affect only surface residues, implying that there are no conformational changes accompanying complex formation.
The phenotypes we observe affect extension of specific branches, but no branches are missing (see Fig. 1).
We did not see any dramatic changes in the distribution of integrin β1, α3 or αV in Rab21-depleted cells (Supplementary Figure 5), nor did we observe affects on cell proliferation following Rab21 knockdown (Supplementary Figure 6A).
The alterations observed affected the whole muscle tissue.
The losses or deletions observed affected principally chromosomes carrying genes encoding enzymes involved in purine metabolism.
Under the same conditions, MAPK and AKT phosphorylations were not, or very slight when observed, affected in OVCAR-3LTE cells.
Prolapse was also observed affecting the NCC of affected horses using 4D echocardiography, whilst other cusps appeared normal.
Hypoglycemia and hypocalcemia was also observed affecting the KO mice.
In the 1990s a new form of CJD was observed affecting mainly young people [ 6].
Uterine fibroids are commonly observed, affecting >30% of women of reproductive age [ 1].
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