Standard curves represented the cycle threshold value as a function of the logarithm of the number of copies generated, defined arbitrarily as 1 copy for the nondiluted standard.
For the global phylogeny, a concatenated alignment of all 272,365 core genome SNPs was generated (defined as genome positions conserved in ≥99% of the genomes).
Using these co-dominant markers, linkage maps that have been generated define ∼500 mapped positions.
To minimise series effects, a computerised random numbers table was generated, defining the order of testers, leg tested, testing position and knee joint position.
For each bioreactor, a heat map was generated defining the differences of community structure on the basis of the annealing temperatures selected for PCR.
Here we describe a method to generate defined chromosomal rearrangements using the Cre-loxP recombination system based on a published strategy [R. Ramirez-Solis, P. Liu, and A. Bradley, (1995) Nature378, 720 724].
We used several means to generate defined deletion and deletion-insertion mutations leaving behind no DNA scars or antibiotic-resistance markers by using suicide plasmid-based technologies [55], [56] and a two-step recombination method [57].
We investigated this phenomenon in detail by generating defined low-quality sequence data.
Encouraged that the CRISPR RNA/Cas9 system functioned in Drosophila, we next asked whether Cas9-mediated DSBs could be harnessed to generate defined deletions.
This study suggests that coupled with soluble factors, PDMS substrates could potentially be useful in generating defined populations of differentiated cells.
