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Each virtual patient was titrated on the simulator with the 4 protocols.
The harvested virus was titrated on Vero cells and the titers of harvests were calculated as plaque forming units/ml (pfu/ml).
Titer was titrated on 3T3 cells.
The reporter strain was titrated on SD/-His plates with varying amounts of 3-AT (0–60 mM) to determine the optimal concentration of 3-AT for background suppression (Matchmaker One-Hybrid System, Clontech).
Thus, to determine the minimum percentage of myoblastsFLPe that still leads to a measurable fusion-dependent signal output, we set up an experiment in which the frequency of myoblastsFLPe was titrated on a constant total amount of 2×105 myoblasts.
The interaction of Zta with the sites was quantitative, as demonstrated by the reduction in complex formation as the Zta protein concentration was titrated on each of the methylated promoters (Figure 5).
Similar(42)
There are case reports of rapid dose titrations occurring safely, however these involved small numbers of acutely unwell patients, being titrated on quetiapine [ 29] and olanzapine [ 30].
Viral stocks were titrated on TZM-bl cells as described previously [39].
Cell-free virus stocks were titrated on FAB cells as described [17].
Then, cells were lysed at 48 h p.i. and infectious particles were titrated on fresh HeLa cells by IFUs assays.
The infectious particles (EBs) released were titrated on fresh HeLa cells by counting the Inclusion Forming Units (IFU) 24 h later.
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