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Furthermore, all PCR amplicons produced in different analyses were subject to verification via sequence analysis.
These repeated sequences can be problematic by complicating sequence verification via routine sequencing technology and could also lead to unwanted partial gene deletion via homologous recombination.
Accuracy of product was determined via sequencing.
UPPAAL is a toolbox for validation (via graphical simulation) and verification (via automatic model-checking) of real-time systems.
To specifically investigate the promoter methylation status of cyclin D2, an initial screen for cyclin D2 promoter methylation was performed by MSP, followed by verification via bisulfite sequence analysis.
Verification of correct sequence and frame was obtained from forward and reverse automated fluorescence sequencing.
After verification by sequencing, all constructs were transformed into Agrobacterium strain GV3101.
After verification by DNA sequencing, pVE5616 was transferred into L. lactis NZ9000.
Pseudogenes were identified by direct comparison with homologs and subsequent verification against original sequencing data.
We thank Mr. Meisheng Zhou for assistance in verification of sequence diversity.
From verification of sequence information of predicted SFP conducted in two independent datasets viz.
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