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Validation of the off-line MEPS/HPLC-DAD method for the determination of six tricyclic antidepressants and their metabolites in human serum was performed.
b, Validation of the p63KO line using western blotting.
For validation of the cell line for caspase activation detection, wide-field microscopy with single excitation for ECFP and dual emission of ECFP and EYFP was collected in ratio mode using an automated fluorescence microscope.
The construction and validation of the cell line HT-29 PPARγ knockdown (HT-29 ShPPARγ) and its negative control (HT-29 ShLuc) are described in Supplementary Materials and methods, available at Carcinogenesis Online.
Therefore, the off-line data generated for validation of the on-line setup can be used to extract the optimal electrochemical reaction conditions for both setups.
Histone modifications were assessed on 132 tumour tissues and 50 normal tissues included in the validation cohort of the LINE-1 study and present on a tissue microarray (described below).
Given the extensive previous validation of the 3T3-L1 celineine as a model of adipocytes24, we used these cells as our model system for the majority of our biophysical and functional characterization of this pathway.
Validation of the refined model.
"Validation of the UK Met.
Validation of the spatial filtering of oligos.
b, Western blot validation of the engineered cell lines.
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