Sentence examples for Upon est from inspiring English sources

At a minimum, readers are advised to use RT PCR to validate differential expression based upon EST hit number prior to conducting downstream experimentation.

In the Cupressaceae, molecular research has largely focused on Cryptomeria japonica, and molecular maps based upon EST, RFLP, RAPD, and isozyme markers exist for this species [ 14- 16].

P. orientale and P. flaccidum were also closely related based upon EST- microsatellite data, even though the P. orientale cytotype examined was 2n = 2x = 18 and likely sexual [ 41], as opposed to the 6x apomictic cytotypes that we studied.

These findings indicate that automated searching for the best predicted methionine start site and removing truncated N- and/or C-terminal tryptic peptide sequences in predicted ORFs based upon EST data increases the ability of MS/MS spectra annotation software to identify peptide sequences with high statistical confidence.

The pairs GBS3230/GBS3231, GBS3172/GBS3173 and GBS3154/GBS3155/GBS3228 each are based upon different EST clusters but represent the same gene as they do not overlap due to shorter contigs, and mapped to a single chromosome bin (Additional files 3 and 4).

Users can therefore view and navigate genomic scaffolds, which include information for gene annotations, gene structure (based upon OGSv1), ESTs and PXS genes.

ICME was founded in 2004, building upon the Scientific Computing and Computational Mathematics Program (est. 1989).

Based upon the preliminary EST sequence data, we estimate that ~78% of these spots represent unique genes (Table 1).

Consequently, a number of groups have developed Brassica cDNA-based microarrays, but these have been based upon relatively modest EST collections and none are available as community resources.

This percentage is comparable to that found previously in our work targeting Arabidopsis hypothetical proteins, and higher than the 21.8% of Arabidopsis genes recently reported to be alternatively spliced by Wang and Brendel based upon large scale EST analysis [ 32].

Based upon estimates from EST and FLcDNA sequencing in three species of spruce, the TPS gene family in conifers appears to be at least of comparable size to those found in angiosperms with sequenced genomes.