HTS data is highly non-uniform in coverage which is influenced by GC content of the underlying sequence where both very high and very low GC content DNA sequence is captured by fewer HTS reads (Dohm et al. 2008).
The coating is uniform in thickness and coverage, and exhibits excellent adhesion to the substrate.
Uniform surface coverage with one thiol was created by incubating a gold surface for 24-72 h in this thiol solution.
From the simulation, it can be expected that low plasma power will result in uniform coverage.
Deposition of Co-Pi or Ni-B on the ZnO surface resulted in uniform coverage along the entire length of the nanowires.
In an effort that spanned several years and all 10 UC campuses, dozens of administrators from across the state worked together to develop an innovative and unified benefits plan for all the vast system's postdocs, including first-in-the-nation uniform health coverage that went into effect on 1 January 2005.
PCR can lead to amplification artefacts that can be alleviated, at least in part, with advanced lab protocols [ 22] which would result in more uniform coverage than what was achieved in this study.
Optimization of the primers used in the multiplex PCR resulted in a uniform coverage distribution over the amplicons that allows for efficient pooling of samples in a sequencing run.
However, this coverage is not uniform- there are wide disparities in coverage among countries and pockets of poor coverage remain within countries with overall coverage above 80percentt.
Templates can be prepared for the PacBio RS II without PCR amplification, resulting in more uniform sequence coverage across genomic regions regardless of GC content, facilitating the detection of minor variants in heterogeneous samples.
Though HTS platforms provide relatively uniform coverage in many standard sequencing experiments, in some of the more challenging applications, such as transcriptome sequencing, the coverage remains drastically uneven (Li et al., 2010; Qu et al., 2009).
