Sentence examples similar to Underline restrictions from inspiring English sources

Underline: restriction sites for BsrGI, NcoI and NotI.

* Lower case: overlap sequences used in SOE PCR; Underline: restriction recognition sequences.

A 1.6 kb OsPIE1 promoter fragment was amplified by PCR using two primers: OsPIE1PF, 5'-GTGTGCTGCAGTGGGTAGAAGGGTAATTGAGAGC-3' and OsPIE1PR, 5'-CCATGTCTAGACTTTCCGAT ATTTTGTAGAGAACTATCT-3', with the Pst I and Xba I (underlined) restriction sites incorporated, respectively.

Underline denotes restriction site.

The M1 cDNA was PCR-amplified using the primers: F-M1-Sma I, 5' TCC CCCGGG CCACCATGAGCCTT CTG ACC GAG GTC 3' and R-M1-Xba I, 5'-TTACT TCTAGA TTACTTGAATCG CTG CAT CTG 3' (underline denotes restriction enzyme recognition sites).

Underlines indicate restriction enzyme sites.

In bold, the overlapping sequences for chimerical constructs and underlined the restriction sites used for cloning.

All plasmids used in this work are listed in Table 1, and the primers used to amplify the ' AcTesA gene are listed in Table 2. Underlines indicate restriction enzyme sites or mutagenized codons.

The primers for pEGFP vector are 5-gg agatctaacatggtgcggactaaag-3 and 5-gg gtcgaccattctttttcatcatttg-3; the primers for pET-32a are 5-gg ggatccgcaacatggtg cggactaaag-3 and 5-gg ctcgagttctttttcatcatttg-3. The sequence underlined is restriction site for subcloning.

To make an inducible tagged copy of TcSOD A, the gene (>Tc00.1047053509775.40 [ 49]) was amplified from genomic DNA of the CL-Brener strain using the following primers: SOD A F: ggg ggatccATGTTGAGACGTGCGGTGAA SOD A R: ggtt gatatcTTTTATTGCCTGCGCAT where underlining indicates restriction sites introduced for ease of cloning.

A 42-bp oligonucleotide sequence containing three tandem repeat copies of 5'-GGA CABREGGCG-3' (ABRE is underlined) and restriction sites of EcoRI and SacI was synthesized and cloned into multiple cloning sites upstream from the HIS3 minimal promoter in the pHIS2 expression vector that had been digested with EcoRI and SacI to get the reporter vector pHIS2-ABRE.