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Z-contrast images and compositional line scanning profiles are acquired by high-angle annular dark-field (HAADF) detector and EDX under STEM mode.
Fig. 6 HAADF images of Au-Ag hollow particles with different rotation angles under STEM mode Fig. 7 Outer diameters and inner diameters of the Au-Ag nanorings.
Analysis was done under STEM mode on a field emission gun TEM FEI Tecnaii G2 F30) equipped with an EDS detector.
This suggests that PPARγ−/− TSC preferentially differentiate to TGC even under stem cell conditions (in TSMFH media).
Moreover, culturing the capsule tissue under stem cell conditions gave rise to colonies that were identical in morphology to the injected cells (data not shown).
This suggests that when maintained under stem cell-specific conditions, the isolated CSC population were maintained in the third passage.
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Cells were cultured as spheres under stem-cell conditions.
To substantiate this finding we also cultivated ex vivo derived BM cells of the Stat5 Δ/+ transplanted animals under stem-cell-supporting conditions (Zhang & Lodish, 2005).
We observed a marked positivity for EGFR within spheres and single cells growing under stem-permissive/serum-free conditions indicating that EGFR immunopositivity observed in tissue sections was retained after CSC enrichment in vitro.
The isolated PROM1/CD133+ cells were cultured under stem-cell conditions in serum-free DMEM/F12 medium (Gibco, 12500-062) supplemented with 20 ng/mL of human recombinant epidermal growth factor (EGF; PeproTech, AF-100-15), 10 ng/mL of FGF2 [fibroblast growth factor 2 (basic)] (bFGF; PeproTech, 100-18B), and 1% N-2 supplement (Gibco, 17502-048).
Also, simplified light calculations in LandMod resulted in the under-prediction of stem growth under canopy structures induced by certain thinning strategies.
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