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Unbound materials were washed from beads complexed with probe set and mRNA by alternating filtration and the addition of wash buffer (0.1X SSC, 0.03% lithium lauryl sulfate).
Unbound materials were removed, and resins were washed three times with ice-cold 0.1% digitonin in TBS before elution with Laemmli sample buffer and heating to 65 °C for 5 min. Eluates and protein-normalized start lysates were subjected to 4 to 12% Bis/Tris sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) for Western blotting.
Two material models, one for asphalt-concrete and another for unbound materials, have been calibrated to laboratory test data.
One of the main applications of recycled aggregates is the construction of sub-bases for roads as unbound materials, substituting for natural aggregates.
This paper evaluates the lab and in situ mechanical properties of non-selected mixed recycled aggregates from construction and demolition waste (CDW) used as base and subbase unbound materials.
After 10 min of incubation at 37°C, the beads were washed to remove unbound materials.
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The membrane was washed to remove unbound material followed by incubation with a cocktail of biotinylated detection antibodies.
Recycled aggregates from crushed concretes (RCA) are usually used as unbound material in road structural layers.
This chapter introduces the basic technical requirements for unbound granular materials and end products, practical uses for slag as an unbound material, and recent research development.
Briefly, GtfB adsorbed to sHA beads were mixed with PI (at concentrations ranging from 0.0025 to 2%) or PBS control, and then washed to remove excess or unbound material.
Parametric analysis was conducted considering the effects of dynamic analysis, temperature gradient, bedrock depth, asphalt layer delamination, viscoelasticity, and unbound material nonlinearity on pavement surface deflections and critical strain responses.
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