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Massively barcoded ChIP-Seq, bearing constant improvements in ultra-throughput sequencing technologies, will become widely accessible and is expected to supplant array-based technologies for gene regulation and expression studies in organisms with both small and larger genomes.
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The sequencing libraries were prepared from the ChIP and Input DNA samples using the Illumina (San Diego, CA) TruSeq DNA Sample Preparation kit following the manufacturer's instructions, and DNA was subjected to ultra-high throughput sequencing on a Illumina HiSeq 2000 DNA sequencers.
Sequencing and library construction was done at the Centre for Ecological and Evolutionary Synthesis' Ultra-high Throughput Sequencing Platform (University of Oslo, Norway) using the 454 Genome Sequencer FLX System (Roche Applied Science).
So, having helped invent an ultra-high-throughput sequencing machine, I used it to sequence my genome.
Quantitative analysis of focused a-to-I RNA editing sites by ultra-high-throughput sequencing in psychiatric disorders.
We demonstrate ultra-high-throughput sequencing of >50,000 cells per run in a synthetic community of Gram-negative and Gram-positive bacteria and fungi.
P. Li, L. Ponnala, N. Gandotra, L. Wang, Y. Si, S.L. Tausta, T. Kebrom, N. Provart, R. Patel, C.R. Myers, E. Reidel, R. Turgeon, P. Liu, Q. Sun, T. Nelson and T.P. Brutnell, "The developmental dynamics of the maize leaf transcriptome as revealed through ultra high-throughput sequencing", Nature Genetics 42(120101060-7 (2010).
The emerging ultra-high-throughput sequencing technology will enable us to obtain whole-genome information, which will be mapped and compared with these references, in less time.
The method presented is equally applicable to transcriptome data generated from ultra high-throughput sequencing (UHTS) approaches.
This study is the first ultra-high-throughput sequencing of miRNAs in the physiologically normal human stomach.
Ultra high-throughput sequencing addresses some of the problems encountered with previous methods of AS detection [12].
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