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Left and right kidney from each animal were homogenized using a Tissue Lyser (Qiagen, TissueLyser Type mm 301), UV radiated 2 metal beats (3 mm), and 30 HZ in 2 min. 100 µl of 10 fold dilutions (range undiluted – 1∶1000) of the tissue homogenate was used to inoculate Sabouraud agar and colonies were counted at day 2 for CFU determination.
Lab equipment and reagents were UV radiated and all lab areas and surfaces wiped regularly with bleach or HCl.
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Figure 2 Comparison of the UV intensities radiated at different microwave intensities Figure 3 Photographs of the electrodeless UV lamp (a) and microwave-discharged lamp set in the microwave oven (b).
The results show that bacteria inhibition efficiency in UV-A radiated TiO2 film groups is significantly higher than that of UV-A radiated only groups.
To form the polymer walls, UV light was radiated for 400 s through a photomask comprising 300 × 300 μm2 of dark square patterns with a 30-μm spacing.
UV radiation readily penetrates cloud cover.
Strong UV radiation damages DNA.
Avoid UV radiation.
Then it was radiated to UV light (broadband 290-385 nm, 12.22 mW/cm2) for 10 mins.
In this research, fish bacteria pathogens, Streptococcus iniae and Edwardsiella tarda, are deposited on TiO2 film and radiated with 500 μW/cm2 of ultraviolet A (UV-A, 360 nm).
As observed for heat stress, the effect of NT219 on the survival of UV-radiated animals was less prominent than that of daf-2 RNAi (average survival 13.89 ± 0.41 days).
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