FISH analyses were carried out using the HER-2 Probe (Oncor, Gaithersburg, MD, USA) and BenchMark XT system.
The level of H2O2 was analyzed by a commercially available Kit (Beyotime Institute of Biotechnology, China) and measured the absorbance at 560 nm using a microplate reader (BioRad-Benchmark, USA).
Rats were anesthetized with 10% chloral hydrate diluted in physiological saline (3.5 ml/kg, IP) and placed into a stereotaxic apparatus (Benchmark, USA) with head held horizontally.
HER-2 status in IHC was evaluated with A485 polyclonal antibody (Dako, Glostrup, Denmark) or 4B5 monoclonal antibody (Ventana Medical Systems Inc., Tucson, AZ, USA) on the BenchMark XT system (Ventana Medical Systems Inc).: biopsies were graded according to the HercepTest (Dako) scoring system (0+, 1+, 2+, or 3+).
OSS was applied to confluent cell cultures using a low-speed orbital shaker (Benchmark Scientific, USA).
Mechanical stress was applied on HepG2 cells only, PBMCs only, and PBMCs/HepG2 cocultured cells using an Orbi-Shaker CO2 (Benchmark Scientific, USA).
Briefly, sections were stained with undiluted hybridoma supernatant of BRAFV600E-specific clone VE1 (provided by Andreas von Deimling, now commercially available at Spring Bioscience, Pleasanton, CA, USA) on a Ventana BenchMark XT immunostainer (Ventana Medical Systems, Tucson, AZ, USA).
Although the data from the current study were collected some years ago, the study provides insight in prevalence, resistance patterns, and risk factors of enterococcal bloodstream infections in Tanzania, confirming similar trends as seen in Europe and the USA, and sets a benchmark for future studies.
For benchmarking with National Healthcare Safety Network NHSNN, USA), data collection and analysis were carried out in accordance with NHSN recommendations.
Indirect immunoperoxidase staining was carried out on four-micrometer-thick sections using an automated stainer (Ventana Benchmark LT, Tucson, USA).
All IHCs were performed using commercially available detection kits and automated membranous staining techniques (Benchmark XT, Ventana, USA).
